Miltenyi Biotech – New automated process to simplify the manufacturing of gene-engineered NK Cells and Clinical-grade tumor-reactive T cell
June 20, 2023
Natural killer (NK) cell and CAR NK cell-based immunotherapies show an enormous potential for the treatment of several diseases. However, the isolation of NK cells can be challenging, and achieving a high purity of the NK cell population is an all-important first step. Clinical-grade natural killer (NK)
cell isolation can be enabled in a two-step,
fully automated manner within a
closed system on the CliniMACS Prodigy. The LP-3-56 Process allows fully automated
in vitro depletion of CD3 expressing cells with optional subsequent
in vitro enrichment of human CD56 expressing cells Following this, isolated NK cells can either be applied directly, expanded for further use, or serve as starting material for NK cell engineering.
The
CliniMACS Prodigy® Tumor Reactive T (TRT) Cell Process offers a new, adaptable platform for scientists and clinicians working on
tumor-infiltrating leukocyte (TIL) therapy, and can be tailored to the individual user’s desired indication and methodology. The TRT process covers the entire workflow:
T cell stimulation, selection of tumor-reactive T cells (by CD137 activation marker) and then expansion them in a closed, automated, GMP-compliant system in as little as two weeks. All cell processing steps are automated to ensure a highly reproducible and standardized manufacturing process and include an optional viral transduction. The TRT standard process offers a high level of flexibility: Starting material for the process can be tumor digest, peripheral blood-derived leukapheresis product, or previously outgrown TILs. Furthermore, specific tumor-reactive T cell populations can also be isolated with GMP-compliant cell sorting using
the MACSQuant® Tyto® Cell Sorter.