New automated process to simplify the manufacturing of gene-engineered Natural Killer Cells and Clinical-grade Tumor-Reactive T cell
June 06, 2024
CAR-engineered NK cells represent a breakthrough in cancer immunotherapy, offering a host of advantages. These include multiple cytotoxicity mechanisms, a decreased risk of graft-versus-host disease (GVHD) in allogeneic scenarios, and minimal associated toxicities. Unlike therapies requiring donor matching, NK cell-based treatments, especially CAR NK cells, can be derived from various cells sources, paving the way for the creation of allogeneic and off-the-shelf products. This accessibility potentially renders them a more cost-effective and readily available treatment alternative. The efficacy of CAR NK therapy has been demonstrated in numerous phase I/II clinical trials, showcasing its potential across a spectrum of cancer types.
Simplifying the manufacturing of CAR NK Cells, The
CliniMACS Prodigy® Natural Killer Cells Transduction (NKCT) Process streamlines and standardizes the production. Covering everything from
cell selection (depletion of CD3 expressing cells with the option of enrichment of human CD56 expressing cells) and activation to viral transduction and expansion, all steps occur within a closed system, ensuring consistent results and reducing operator hands-on time.
The
CliniMACS Prodigy® Tumor Reactive T (TRT) Cell Process introduces a versatile platform for researchers and clinicians focused on
tumor-infiltrating leukocyte (TIL) therapy, adaptable to specific indications and methodologies. It covers the entire workflow:
T cell stimulation, selection of tumor-reactive T cells using the CD137 activation marker) and expansion in a closed, automated, GMP-compliant system, all within just two weeks. Every cell processing step is automated, ensuring a highly reproducible and standardized manufacturing process, with an option for
viral transduction. The TRT standard process is highly flexible, accommodating starting materials such as tumor digests, peripheral blood-derived leukapheresis products, or previously cultured TILs. Additionnally, specific tumor-reactive T cell populations can be isolated using GMP-compliant cell sorting using
the MACSQuant® Tyto® Cell Sorter. Our technologies facilitate the development of easy, safe, and effective personalized T cell therapies.